superresolution fluorescence microscope  (Evident Corporation)

Journal: Science Advances

Article Title: Wax-wetting sponges for oil droplets recovery from frigid waters

doi: 10.1126/sciadv.abc7926

Figure Lengend Snippet: ( A ) Light microscope images show droplet agglomeration and coalescence with decreasing temperature. ( B ) Polarized microscope images show the crystallized wax accumulated at the surface and interface of the oil droplets. ( C ) The fluorescence images show that the amount of water (black) entrapped inside the oil (green) droplets has increased with decreasing temperature. At lower temperatures, because of wax crystallites and water entrapment, the oil droplets transformed from stable oil droplets (right) to double emulsions (middle) and a viscous gel (left). Background colors represent temperature regimes at approximately 5°C (white), 20°C (blue), and 40°C (red), where the waxes are in solid, liquid, and dissolved phases, respectively. Scale bars, 50 μm (A and B) and 2 μm (C).

Article Snippet: The crude oil (green) and water (black) phases of the emulsified crude oil droplets were differentiated using a confocal fluorescence microscope (ZEISS LSM 880 Elyra Superresolution Imaging System).

Techniques: Light Microscopy, Microscopy, Fluorescence, Transformation Assay

Journal: Applied and Environmental Microbiology

Article Title: Magnetotactic Bacteria Accumulate a Large Pool of Iron Distinct from Their Magnetite Crystals

doi: 10.1128/AEM.01278-20

Figure Lengend Snippet: Red (left panels), green (center panels), and merged (right panels) fluorescence images obtained from superresolution microscopy of wild-type, Δ mamP , and Δ mamT AMB-1 incubated with FIP-1 for 180 min. The two Δ mamP panels show the two fluorescence patterns (diffuse and located to the magnetosome chains, as in wild-type) observed in the populations. Scale bars = 4 μm. Additional pictures are available in the supplemental material.

Article Snippet: All bacterial samples were observed by structured illumination microscopy with a Carl Zeiss Elyra PS.1 superresolution fluorescence microscope, using red (excitation wavelength of 561 nm, emission wavelength of 570 to 620 nm) and green (excitation wavelength of 488 nm, emission wavelength of 495 to 550 nm) laser lines for the detection of the native and cleaved probe, respectively.

Techniques: Fluorescence, Microscopy, Incubation

Journal: Nature Communications

Article Title: Impaired β-arrestin recruitment and reduced desensitization by non-catechol agonists of the D1 dopamine receptor

doi: 10.1038/s41467-017-02776-7

Figure Lengend Snippet: Non-catechol D1R agonists fail to induce desensitization via impaired β-arrestin recruitment. a Schematic of agonist-stimulated desensitization of GPCRs. Prolonged activation reduces subsequent agonist-induced signaling. b Experimental design to test for agonist-stimulated D1R desensitization. c Agonist-induced (1 μM SKF-81297) cAMP elevation and percent desensitization of D1 receptor signaling in striatal neurons treated for 120 min with vehicle or 10 μM of the indicated agonists. One-way ANOVA revealed a significant effect of treatment on cAMP levels, F (9, 108) = 32.1, P < 0.001; post-hoc Dunnett’s comparisons indicated only catechol agonists had significantly different level of cAMP versus vehicle (*** P < 0.001). Percent desensitization was significantly affected by treatment (one-way ANOVA F (8,99) = 24.2, P < 0.0001); Dunnett’s comparisons indicated significantly higher percent desensitization in all catechol groups versus vehicle (*** P < 0.001), while non-catechol groups were not significantly different from vehicle. All data represent means ± s.e.m., n = 3 per group. d Percent D1 receptor desensitization after various times of agonist exposure. Two-way ANOVA revealed significant trends in time (linear F (1,59) = 44.2, P < 0.0001 and quadratic F (1,59) = 8.5, P < 0.005) and significant interaction effect ( F (3,59) = 5.1, P < 0.003); pairwise Tukey post-hoc tests at time points 60,120, and 180 min indicated that catechol and non-catechol groups were significantly different (*** P < 0.001, n = 4 per group; batches are independent from panel c ). e Live cell total internal reflection fluorescence microscopy (TIRFM) imaging of β-arrestin-GFP recruitment to the plasma membrane of U2OS cells expressing human D1R. Images show β-arrestin-GFP at the plasma membrane of cells before and after exposure to the indicated agonists (1 μM). f Quantification of membrane β-arrestin-GFP puncta in the cells shown in e . g TIRFM images of β-arrestin-GFP at the plasma membrane in U2OS cells after 10 min of treatment with indicated agonists (1 μM). Scale bars, 10 μm. h Quantification of β-arrestin-GFP membrane recruitment via total signal per cell from ≥60 cells per group obtained across three independent experiments. One-way ANOVA revealed that treatment had a significant effect ( F (9,33) = 130.9, P < 0.0001; Dunnett’s comparisons with dopamine group indicated significantly lower total signal in vehicle, PF-1119, PF-8294 (*** P < 0.001) and PF-6142 (* P < 0.05), but not in PF-2334, and not for all catechol agonists groups. All data represent means ± s.e.m., n ≥ 3

Article Snippet: All images were captured using a Zeiss PS.1 Elyra Superresolution fluorescence microscope equipped with TIRF module.

Techniques: Activation Assay, Fluorescence, Microscopy, Imaging, Expressing